We develop novel optical methods and instrumentation for the observation of biological phenomena with the highest fidelity. In particular, we focus on super resolution fluorescence imaging and single molecule tracking implementations that offer outstanding spatial and/or temporal resolution. By bringing together expertise in optics, electronics, data processing, labelling and photophysics, we develop solutions to transversal obstacles in biology and biophysics such as quantification of protein clusters and complexes, and identification of protein interaction and dynamics.
Figure 1. (A) Principle of PALM/STORM, where the resolution depends on the wavelength, numerical aperture and number of collected photons. (B) MINFLUX depicted as a ruler, where emitters are located from a sequence of exposures to tailored light patterns. The resolution now depends on the size of the ruler (L, separation between sequential excitations), instead of the wavelength. (C) A MINFLUX scheme in 2D with multiple excitations with doughnut-shaped beams, each coloured dot is the center of an exposure. (D) A MINFLUX scheme in 3D, with a beam created by top-hat wavefront shaping. (E) Iterative MINFLUX, where successive approximations to the location of the molecule produce a localization that surpasses the typical N-1/2 dependence..
Figure 2. (A) MINFLUX nanoscopy by sequentially localizing individually blinking fluorophores; a DNA-origami arrangement is imaged. (B) MINFLUX tracking of a DNA-origami flipping device, a fluorophore is followed with 2.5 nm precision every ~0.5 ms. (C) MINFLUX tracking of the small ribosomal subunit protein in living E. coli. (D) Iterative MINFLUX imaging of nuclear pore complex in 3D (fixed) and 2D (live, with comparison to classical SMLM), and of the PSD-95 protein at a neuronal synapse.
Senior Research Assistant
Positions calls are now open (last update 10.02.2020).
Super Resolution Microscopy at the Molecular Scale
MINFLUX: Super Resolution
at the Molecular Scale
MINFLUX: Next generation Super Resolution Microscopy and Single Molecule Tracking
Seminar Series: Modern Concepts in Structural Biology
MINFLUX: Achieving the ultimate resolution limit in fluorescence microscopy
San Francisco, USA
Isotropic 3D molecular resolution in cells with MINFLUX nanoscopy
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